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Biofilm assay on three transconjugants, IG9, IIB3, and IIE7, obtained by filter mating wild-type E99 with E. faecalis strain OG1RF. Crystal violet binding was used to measure the 24-h biofilm densities of IG9, IIB3, IIE7, and parent strain OG1RF grown in TSB supplemented with 0.5% glucose. The error bars represent the mean ± standard error.
