TABLE 1.
E. faecalis strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. faecalis strains | ||
| E99 | Clinical isolate | This study |
| P101D12 | E99 with a Tn917 insertion in bee-2, Ermr | This study |
| BLG1 | P101D12 complemented with pBLG1 expressing bee-2, Spcr | This study |
| BLG2 | P101D12 complemented with pBLG2 expressing bee-3, Spcr | This study |
| BLG12 | P101D12 complemented with pBLG12 expressing bee-2 and bee-3, Spcr | This study |
| OG1RF | Plasmid free, Rifr, Fusr | 5 |
| JH2SS | Plasmid free, Strr, Spcr | 14, 42 |
| POTC-1, POTC-2, and POTC-3 | Transconjugants derived from P101D12 and OG1RF mating, Rifr, Fusr, Ermr | This study |
| POJTC-1, POJTC-2, and POJTC-3 | Transconjugants derived from POTC-2 and JH2SS mating, Strr, Spcr, Ermr | This study |
| IG9, IIB3, and IIE7 | Transconjugants derived from E99 and OG1RF mating, Rifr, Fusr | This study |
| Tn917 mutant strains | ||
| P105H2 | Tn917 insertion in srt-1 | This study |
| P101A12 | Tn917 insertion in srt-1 | This study |
| P101D12 | Tn917 insertion in bee-2 | This study |
| P76D4 | Tn917 insertion in bee-1 | This study |
| P94C6 | Tn917 insertion in bee-1 | This study |
| P110B1 | Tn917 insertion in bee-3 | This study |
| P15D1 | Tn917 insertion upstream of srt-1 | This study |
| P77E12 | Tn917 insertion upstream of srt-1 | This study |
| Plasmids | ||
| pAT28 | Shuttle vector, Spcr | 44 |
| pTV1-OK | Temperature-sensitive plasmid containing Tn917, Kanr, Ermr | 10 |
| pBLG1 | pAT28 containing full-length bee-2 downstream of the aphA-3 promoter, Spcr | This study |
| pBLG2 | pAT28 containing full-length bee-3 downstream of the aphA-3 promoter, Spcr | This study |
| pBLG12 | pAT28 containing both bee-2 and bee-3 downstream of the aphA-3 promoter, Spcr | This study |