FIG. 6.
MprA-dependent induction of mprA and sigE expression requires the extracytoplasmic loop domain of MprB. Wild-type or mprA::Kmr mutants of M. tuberculosis expressing various alleles of mprA and mprB from an integrative plasmid (A) were examined for the expression of mprA (B) or sigE (C) in the absence of stress (open bars) or following exposure to 0.2% Triton X-100 (filled bars). Values are presented as mean differences (n-fold [±standard deviations]) of mprA or sigE mRNA levels from that of sigA mRNA and were determined from experiments performed in triplicate. *, P < 0.05 between untreated and treated cultures.