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. 2003 Feb;185(3):809–822. doi: 10.1128/JB.185.3.809-822.2003

FIG. 2.

FIG. 2.

Bioassay of whole-cell extracts from Rhizobium sp. strain NGR234 derivatives. TLC of dichloromethane whole-cell extracts from various NGR234 derivatives standardized to an OD600 of 1.5 and fractionated on C18 reverse-phase silica plates in a mobile phase of 60% methanol is shown. The plates were overlaid with acyl-HSL-responsive reporter strain A. tumefaciens NTL4(pCF218)(pCF372) as described previously (59, 74). Lane 1, NGR234(pSW213, lacIq)(pBDJ6, Plac-traR) diluted 200-fold. Lane 2, NGR234(pSW213). Lane 3, NGR234-XH100 (traI null pXH100 integrant). Lane 4, NGR234-XH100(pBDJ6, Plac-traR). Lane 5, NGR234-ANU265 (pNGR234a cured). Lane 6, NGR234-ANU265(pBDJ6, Plac-traR). Lane 7, extract of RMM. The vertical arrow indicates the direction of mobile phase flow, and the horizontal arrow marks the point of sample application. Synthetic standards flank sample lanes. Synthetic 3-oxo-acyl-HSL standards (3-O-C) are as follows: 3-oxo-C6-HSL, 0.25 pmol; 3-oxo-C8-HSL, 0.21 pmol; 3-oxo-C12-HSL, 0.25 nmol. Synthetic acyl-HSL standards (C) are as follow: C6-HSL, 0.26 nmol; C8-HSL, 2.5 pmol; C10-HSL, 2.5 nmol; C12-HSL, 2.5 nmol; C14-HSL, 2.5 nmol. The image was prepared with Corel Photopaint 9.0 and CorelDraw 9.0.