TABLE 5.

In vivo assays of traM cross-functionality^a

TraM Plasmid	β-Galactosidase sp act (Miller units)b with:
	No inducer	AAIc	IPTG (1 mM)	IPTG + AAI
pBBR1-MCS5 (vector)d	<5	571	<5	551
pGB200 (Ti traM)	<5	528	<5	32
pCF424 (NGR234 traM)	<5	578	<5	612

^aAll assays were performed with A. tumefaciens NTL4 harboring pCF218 (P_tetR-traR) and pCF372 (P_traI-lacZ).

^bResults are averages from assays in triplicate.

^cWith 0.05% (vol/vol) cell-free supernatant of the AAI overproducer A. tumefaciens KYC6(pCF218), having bioactivity equivalent to 0.4 μM synthetic 3-oxo-C8-HSL.

^dBoth traM expression plasmids are derivatives of pBBR1-MCS5.