TABLE 2.
Sulfur metabolism genes differentially expressed in B. subtilis ΔyrzC (BSIP1793) compared to the wild-type strain (BSIP1215) in the presence of sulfate as the sole sulfur sourcea
| Gene name and synonym | Function/similarity | Transcriptome analysis
|
Met/SO4 regulationb | |
|---|---|---|---|---|
| ΔyrzC/wild-type expression ratio | P value | |||
| cysK | OAS thiol-lyase | 3.21 | 8.10−3 | + |
| ydbMc | Similar to butyryl-CoA dehydrogenase | 1.93 | 4.10−3 | + |
| mtnN (yrrU, mtnA) | SAH/MTA nucleosidase | 2.37 | <1.10−4 | + |
| yrhA | Putative cystathionine β-synthase | 4.28 | 1.10−4 | + |
| yrhB | Putative cystathionine γ-lyase | 3.20 | <1.10−4 | + |
| Transporters and associated genes | ||||
| ytmI | Predicted acetyltransferase | 3.98 | 1.10−4 | + |
| tcyJ (ytmJ) | l-Cystine ABC transporter (binding protein) | 4.27 | 2.10−4 | + |
| tcyK (ytmK) | l-Cystine ABC transporter (binding protein) | 4.82 | 1.10−4 | + |
| tcyL (ytmL) | l-Cystine ABC transporter (permease) | 4.00 | 1.10−4 | + |
| tcyM (ytmM) | l-Cystine ABC transporter (permease) | 3.35 | 1.10−4 | + |
| tcyN (ytmN) | Similar to amino-acid ABC transporter (ATP-binding protein) | 4.49 | <1.10−4 | + |
| ytmO | Similar to monooxygenase | 5.05 | 1.10−4 | + |
| ytnI | Putative glutaredoxin-like protein (GrxC) | 3.34 | 1.10−4 | + |
| ytnJ | Monooxygenase | 4.88 | 1.10−4 | + |
| ribR | Riboflavin kinase | 4.50 | <1.10−4 | + |
| hipO | Hippurate hydrolase | 8.25 | <1.10−4 | + |
| ytnM | Permease-like protein | 5.59 | <1.10−4 | + |
| tcyP (yhcL) | Similar to sodium-glutamate symporter, l-cystine transporter | 2.84 | 1.10−4 | + |
| yxeK | Similar to monooxygenase | 1.67 | 1.10−4 | + |
| yxeL | Predicted acetyltransferase | 1.75 | 1.10−4 | + |
| ssuA (ygbA) | Aliphatic sulfonate ABC transporter (binding lipoprotein) | 3.69 | 1.10−4 | + |
| ssuC (ygaM) | Aliphatic sulfonate ABC transporter (permease) | 2.28 | 1.10−4 | + |
| ssuD (ygcA) | Aliphatic sulfonate monooxygenase | 5.89 | 1.10−4 | + |
| ygaN | Unknown | 3.42 | 1.10−4 | + |
| Sbox family | ||||
| metC (yjcJ) | Cystathionine β-lyase | 0.65 | 3.10−3 | |
| met E | Cobalamin-independent methionine Synthase | 0.62 | 8.10−3 | − |
| yoaB | Similar to α-ketoglutarate permease | 0.48 | 3.10−4 | − |
| mtnB (ykrY) | MTRu-1-P dehydratase | 0.64 | 4.10−3 | − |
| mtnD (ykrZ) | Aci-reductone dioxygenase | 0.64 | 1.10−3 | − |
The results obtained are representative of eight hybridizations from four independent cultures. The data sets generated were loaded into the GenoScript Database (http://genodb.pasteur.fr).
+ and − indicate a higher and a lower expression level on methionine than on sulfate, respectively (1).
The ydbM gene encoding a protein similar to butyryl-CoA dehydrogenase was included in this table. Indeed, the level of ydbM expression was high with methionine and repressed with sulfate (1). ydbM was also found to be a target of YrzC in this study. Its possible role related to sulfur metabolism remains to be determined.