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Effect of Int on cleavage by MBP-Orf20. Left panel: T strand. Right panel: NT strand. Lane 1: DNA probe in the presence of 320 nM Int. Lane 2: 125 nM MBP-Orf20. Lanes 3 to 6: 125 nM MBP-Orf20 with increasing amounts of Int from 40 nM to 320 nM. Lanes 7 to 10: DNA-sequencing reactions.
