TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| GAS | ||
| AL168str-r | M22, OF+, streptomycin-resistant derivative of AL168 | 28 |
| nr4 | M22, Tn916 mutant of AL168str-r in mga | 28 |
| JRS4 | M6, streptomycin-resistant derivative of D471 | 26 |
| JRS4-PolHis (12Z05A) | M6, derivative of JRS4 with chromosomal His-tagged β′ subunit of RNA polymerase | 21 |
| JRS519 | M6, derivative of JRS4 (mga-10) | 18 |
| SF370 | M1, sequenced strain | 27 |
| AP4 | M4, OF+ | 14 |
| E. coli | ||
| DH5α | hsdR17 recA1 gyrA endA1 relA1 | 9 |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | Novagen |
| Plasmids | ||
| pBluescript II KS(−) | ColE1 ori AmprlacZα | Stratagene |
| pCD2 | PT7-σA (Bacillus subtilis) | 5 |
| pKSM164 | Pmga-mga-his | 1 |
| pKSM170 | PT7-mga-his | 1 |
| pKSM415 | PrpsL, 3′ HindIII site | This study |
| pKSM416 | Pemm, 3′ EcoRI site | This study |
| pKSM417 | PsclA (sequence downstream of Mga-binding site), 3′ XhoI site | 1 |
| pKSM418 | PsclA (ΔMga-binding site), 3′ XhoI site | 1 |
| pKSM419 | PsclA (full length), 3′ XhoI site | 1 |
| pKSM420 | Psof, 3′ BamHI site | This study |