TABLE 2.
PCR primers used in this study
| Target | Primer | Sequence (5′-3′) | Reference |
|---|---|---|---|
| Nonspecific | rpsL-1 | GAATGTAGATGCCTACAATTAACCA | 17 |
| rpsL-2 | GTGCGCCACGAACGATATG | 17 | |
| Pemm | Pemm-L1 | GCATGGATCCCATCGCAAAGAGCTTA | 17 |
| Pemm-R1 | GCGGCTCGAGTAGTGTCTATTCGTGTTATT | 17 | |
| Pemm1 | Pemm1-L1 | AAGCGATTATTGACAAGCTC | 1 |
| Pemm1-R1 | AGTCAAAGCTACCGCTACTG | 1 | |
| Pmga | OYL-25 | TACCATAAAATACCTTTC | 17 |
| OYR-25 | GGTTGTACCATAACAGTC | 17 | |
| PrpsL | GAS-rpsL1 | TGTCTAAAATCACATCTTCGA | This study |
| GAS-rpsL7 | TTACGTACCAACTGGTTAATT | This study | |
| PsclA | PsclA-L2 | TGGTAATCGTAATTGTCTGC | 1 |
| PsclA-R | ATGGTGCTTTGATGTCAACA | 1 | |
| PscpA | C5-L1 | AAGAATGAGATTAAGGAGGTCACA | 16 |
| C5-R1 | GCGCAATGGCAAGTTTGTC | 16 | |
| Psof | Psof-L3 | TTTGGTCTCAGACGGCGCCA | This study |
| Psof-L4 | AATCAAGGTCACAAACTTTC | This study | |
| Psof-L5 | AATCGTCTGATAAGCTCTTG | This study | |
| Psof-L6 | CACTCAGTTGCCTCCAAGCT | This study | |
| Psof-R1 | ACCTCCTGTCCTAAAACTGT | This study | |
| Psof-R2 | GAAAAAAGGGGTTGCCGCGA | This study | |
| Psof-R3 | GTCAATGTTTCGTTAACCTT | This study | |
| Psof-R4 | GGAAAGTTTGTGACCTTGAT | This study | |
| Psof-R5 | CGTCTGAGACCAAAGCGTTA | This study | |
| Psof-R6 | GCTGCGCAAGGAGAAGCTCA | This study |