TABLE 2.

ATP- and Mg²⁺-dependent association of H-MinD with phospholipid vesicles^a

Nucleotide	% Content in pellet
	Without lipid, H-MinDb	With lipid
		Lipidb	H-MinDb	H-MinD
ATP	13	93	51	55
ATPγS	7	97	42	43
GTP	11	98	27	27
dATP	5	98	24	24
ADP	10	97	21	21
ATP (no Mg2+)c	NDd	92	19	21

^aH-MinD (2.0 μM) was added to mixtures containing phospholipid vesicles (0.5 mg/ml) and nucleotide (500 μM) in RB. MgCl₂ was added to 1 mM, and mixtures were incubated for 15 min at 30°C and then subjected to centrifugation for 1 min at 16,000 × g. The percentages of H-MinD and phospholipid recovered in the pellet fractions were measured as described in Materials and Methods. The last column shows the percentages of H-MinD in the pellet fractions after adjustment for the incomplete sedimentation of phospholipids.

^bMeasured values.

^cMgCl₂ was omitted from the reaction.

^dND, not detected.