TABLE 4.

MinE-H stimulated dissociation of H-MinD from predecorated vesicles^a

Reaction	Condition	Mean % protein (SD) in pellet at:
		1 min			25 min
		H-MinD	MinE-H	*MinE-H	H-MinD	MinE-H	*MinE-H
1	Spin 1	66.0 (1.0)			67.5 (1.5)
2	P1 + buffer	82.0 (1.0)			87.0 (2.0)
3	P1 + MinE-H	50.0 (2.1)	16.3 (1.5)		31.8 (4.3)	4.5 (1.1)
4	P1 + *MinE-H	81.0 (0.0)		0.0 (0.0)	82.0 (2.0)		0.5 (0.5)

^aIn reaction 1, H-MinD (5 μM) was incubated with vesicles (1 mg/ml) in RB plus 500 μM ATP and 1 mM MgCl₂ for 10 min at 30°C. Vesicles were sedimented (16,000 × g for 1 min; spin 1). The pellet (P1) was resuspended in the original volume of RB plus 500 μM ATP and 1 mM MgCl₂, and the percentages of H-MinD and phospholipid were determined. For reactions 2 to 4, resuspended P1 was supplemented with MinE storage buffer (reaction 2), MinE-H (reaction 3), or *MinE-H (reaction 4), and mixtures were incubated for 5 min at 30°C. Vesicles were sedimented (16,000 × g) for either 1 or 25 min, and the percentages of phospholipid, H-MinD, MinE-H, and *MinE-H in the pellet fractions were determined. MinE-H and *MinE-H were used at 5 μM, and the H-MinD/MinE-H and H-MinD/*MinE-H ratios in reactions 3 and 4 were ∼0.67. The mean percent values and standard deviations of two (reactions 1, 2, and 4) or four (reaction 3) independent experiments obtained with two different preparations of H-MinD and MinE-H are given. Values were adjusted for incomplete sedimentation of vesicles. More than 93% of the phospholipid was recovered in the pellet fraction in each case.