FIG. 3.

(Top) Organization of the genes encoding the acyl-CoA carboxylase of A. brierleyi. The accC, accB, and pccB genes encode the BC, BCCP, and CT subunits, respectively. Selected restriction sites are indicated below the filled rectangle (and the open box, representing the partial sequence), as follows: X, XbaI; P, PstI; E, EcoRI. Hatched rectangle, DNA fragment used as a probe for Southern hybridization. Thick bars, cloned and subcloned fragments. (Bottom) Nucleotide sequences of fragments A through D delineated in the diagram above. Stop codons and initiation codons are boldfaced. Termination signals, promoter sequences, and RBSs are underlined.