TABLE 1.

Purification of the A. brierleyi acyl-CoA carboxylase^a

Step	Amt of protein (mg)	Activity (U)b of:		Sp act (U/mg) of:		ACC/PCC ratio	ACC yield (%)	ACC purification (fold)
		ACC	PCC	ACC	PCC
Cell extract	558	340	298	0.6	0.5	1.20	100	1
Streptavidin	4.14	52.5	41.2	12.7	10.0	1.27	15	21.2
Superose 6	1.96	28.4	23.4	14.5	11.9	1.22	8.4	24.2

^aFrom ca. 10 g of cells (wet weight).

^bThe activities of acetyl-CoA carboxylase (ACC) and propionyl-CoA carboxylase (PCC) were measured at 70°C. One unit of enzymatic activity is defined as the amount of enzyme which incorporates 1 μmol of NaH¹⁴CO₃ per min.