Heme utilization by B. bronchiseptica strains. Growth stimulation bioassays with solutions of bovine hemin chloride or human hemoglobin added to wells: A, 50 μM hemin; B, 100 μM hemin; C, 25 μM hemoglobin. Strains: B013N, wild-type B. bronchiseptica parent strain; BRM23, ΔhurI; BRM23(pRK37), BRM23 carrying plasmid-borne hurI.