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Amino acid substitutions in RP4 TrbE and common motifs in the VirB4 protein family. Protein RP4 TrbE is represented by the gray bar. The predicted TMH and motifs A to D are represented by solid boxes. Each of the mutations influenced the Pil, Dps, and Tra phenotypes (Table 2). Proposed consensus sequences of the common motifs found in proteins belonging to the VirB4 family are shown below the bar. aa, amino acid; NBS, nucleotide binding site.
