TABLE 3.
Strains used for trbE complementation
| Strain | Relevant genotype | Phenotypea
|
||
|---|---|---|---|---|
| Pil | Dps | Tra | ||
| JE2571(pMS123) | (trbB to trbM)+ (traF)+ | + | + | NA |
| JE2571(pMS123ΔtrbE) | (trbB to trbD, trbF to trbM)+ (traF)+trbE0 | − | − | NA |
| JE2571(pMS123ΔtrbE, pCR21)b | (trbB to trbD, trbF to trbM)+ (traF)+trbE+b | ? | ? | NA |
| HB101(pDB126) | (trbB to trbM)+ (traF to traM)+oriT+ | NA | NA | + |
| HB101(pDB126ΔtrbE) | (trbB to trbD, trbF to trbM)+ (traF to traM)+oriT+trbE0 | NA | NA | − |
| HB101(pDB126ΔtrbE, pCR21)b | (trbB to trbD, trbF to trbM)+ (traF to traM)+oriT+trbE+b | NA | NA | ? |
a
+, positive; −, negative; ?, depends on the mutation; NA, not applicable. For experimental details see Materials and Methods.
b
Plasmid pCR21 carried either the wild-type trbE allele (pCR21) or a trbE mutation (pCR21X000Z or pCR21Δ1-11) (Table 2).