TABLE 2.
Effect of expression of spoIIIESu after septation on sporulation of B. subtilis spoIIIE mutants
| Relevant genotypea (strain) | No. of heat-resistant spores per mlb
|
Frequency (%) relative to isogenic spo+ strain | ||
|---|---|---|---|---|
| Series 1 | Series 2 | Series 3 | ||
| spo+ (SL8509) | 2.4 × 108 | 1.3 × 108 | 4.8 × 108 | 100 |
| spoIIIE36 (SL8776) | <10 | <10 | ND | <10−5 |
| spoIIIE36 PspoIID-spoIIIESu (SL8808) | 8.9 × 106 | 4.9 × 106 | 5.3 × 106 | 1-4 |
| spoIIIE36 PspoIIR-spoIIIESu (SL10406) | 2.4 × 106 | 3.0 × 106 | ND | 1-3 |
| spoIIIE36 PspoIIQ-spoIIIESu (SL7980) | <10 | 1.2 × 103 | 3.9 × 102 | <0.001 |
| spoIIIE36 PspoIIQ-spoIIIESu; PspoIID-spoIIIESu (SL8816) | 4.9 × 106 | 4.2 × 106 | ND | 2-3 |
| spoIIIE::spc PspoIIQ-spoIIIESu (SL7983) | 5.9 × 106 | 1.5 × 105 | ND | 0.1-2 |
| spoIIIE::spc (SL7375) | <10 | <10 | ND | <10−5 |
a
All strains except SL7375 contain thrC::PspoIIQ-lacZ.
b
Sporulation was assessed 20 h after the end of exponential growth. Heat treatment was for 20 min at 80°C. The results of three separate series of experiments are shown. Relative frequencies are estimated within a series. ND, not determined.