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SDS-PAGE Coomassie-stained gel (A) and Western immunoblot (B) showing the location of SrgA. Strain NLM2198/pLMN107 was grown in the absence (−) and presence (+) of arabinose induction of SrgA. Cells were harvested and fractionated as described in Materials and Methods. WC, whole-cell lysate; P, periplasmic fraction; C, soluble cytoplasmic fraction; M, membrane fraction. Molecular size markers are indicated to the left of the panels.
