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. 2003 Feb;185(3):957–965. doi: 10.1128/JB.185.3.957-965.2003

FIG. 3.

FIG. 3.

RT-PCR analysis of B. burgdorferi relBbu mRNA. (A) Relative concentrations of relBbu mRNA in B. burgdorferi N40 grown in BSK-H medium or in BSK-H medium in rat peritoneal chambers or in B. burgdorferi B31 grown in BSK-H medium. relBbu mRNA was detected by competitive RT-PCR. All reaction mixtures contained 1 ng of B. burgdorferi total RNA. The amounts of competitor were 16, 8, 4, 2, and 1 fg in lanes 1 to 5, respectively. Lane 3 for N40 and lane 4 for B31 showed equal signal intensities for relBbu mRNA (upper band) and the competitor (lower band), indicating that in these reaction mixtures they were present at equal concentrations. In lane C RNA was omitted. (B) relBbu mRNA in B. burgdorferi N40 in BSK-H medium and in ticks. −, reverse transcriptase omitted; +, complete reaction mixture for specific RNA detection.