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. 2003 Feb;185(3):957–965. doi: 10.1128/JB.185.3.957-965.2003

FIG. 6.

FIG. 6.

Complementation of E. coli CF1693 (relA− spoT−) with B. burgdorferi relBbu homologue. (A) E. coli CF1693 was transformed with pBluescript IISK+ (left side) or pBlue-ST plasmid DNA (right side) and grown on a 1.5% agar-M9 medium plate. (B) B. burgdorferi relBbu mRNA synthesis by CF1693 containing pBlue-ST following growth in MOPS starvation medium or LB medium. −, reverse transcriptase omitted; +, complete reaction mixture for specific RNA detection. In lane C RNA was omitted. (C) (p)ppGpp accumulation in E. coli, as determined with wild-type strain CF1648 and the CF1693 relA− spoT− double mutant containing pBlue-ST in MOPS starvation medium or LB medium.