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Primer extension from cipC, cel48F, cel8C, cel9G, and cel9E mRNAs. The experiments were performed with 15 μg of total RNAs and primers covering the 5′ end of the various ORFs (cip-PE1 for cipC, F-PE1 for cel48F, C-PE1 for cel8C, G-PE1 for cel9G, and E-PE1 for cel9E) The sizes of the reverse transcripts were determined by using a labeled 25-bp DNA Step Ladder (Promega).
