TABLE 1.
Primer | Sequencea |
---|---|
TLPA-P1 | 5′-AACAAGCTCGCTAAAGGCTG |
TLPA-P2K | 5′-TTGGCGTATAACATAGTATCGTCA CAATCAACGCCACGCAC |
TLPA-P3K | 5′-TAGTACCTAGATTTAGATGTCATC GGTTACTGAGGGCAATC |
TLPA-P4 | 5′-AAAGCTCGTTTTCTCTCGCC |
TLPB-P1 | 5′-TGGTTACAGACGCTGATAGG |
TLPB-P2K | 5′-TTGGCGTATAACATAGTATCGAAC ACGACCAGCATGATACG |
TLPB-P3K | 5′-TAGTACCTAGATTTAGATGTCATG TGAGTGGAACGACCATG |
TLPB-P4 | 5′-CAAGCGGATGATCATCTC |
TLPC-P1 | 5′-ATCGCTAAAGTGTGGCTCAC |
TLPC-P2K | 5′-TTGGCGTATAACATAGTATCGTAA CAACCGCACACACCATC |
TLPC-P3K | 5′-TAGTACCTAGATTTAGATGTCGAA GATGTGAGCAGGAAG |
TLPC-P4 | 5′-TGAACGACGATATGGGAGAC |
TLPD-P1 | 5′-GTGTGCATCCATGAATAAGA |
TLPD-P2C | 5′-CCCAGTTTGTCGCACTGATAAGGA AGATAGTGCTAAACATG |
TLPD-P3C | 5′-ATCCACTTTTCAATCTATATCTGT TCAAGCTTGCAAGATTCC |
TLPD-P4 | 5′-CTTTAATCATTACACACCGC |
CATF-E1 | 5′-GATATAGATTGAAAAGTGGAT (cat) |
CATR-E2 | 5′-TTATCAGTGCGACAAACTGGG (cat) |
KAMF-E1 | 5′-CGATACTATGTTATACGCCAA (aph3-A gene) |
KAMR-E2 | 5′-GACATCTAAATCTAGGTACTA (aph3-A gene) |
RDXFSac | 5′-CACGAGCTCTGGTAATTGTTTCGTTAGGG (5′ rdxA) |
RDXRXba | 5′-CACTCTAGACTTTATAAGACTCCGGATAG (5′ rdxA) |
RDXFXho | 5′-TTGCTCGAGTGCTTGGCG (3′ rdxA) |
RDXRKpn | 5′-ATCGGTACCAAGTAATCGCATC (3′ rdxA) |
TLPBCompF | 5′-GCAGGATCCTCAAAGGATGGGAGGACTT |
TLPCompR | 5′-GCAGAATTCACGGAAAGAATGGTGTCTTC |
Restriction endonuclease cleavage sites are underlined.