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Confocal laser scanning microscopic images of (A) 6-day and (B) 9-day-old Streptococcus pneumoniae serotype 3 biofilms. Biofilms were grown in flow cells under once-through flow conditions for 6 and 9 days, after which time the biofilms were stained with the Live/Dead BacLight stain. Biofilms were viewed at 400× magnification. Flow cell experiments were performed in triplicate as described in Materials and Methods.
