TABLE 3.
Quantitative analysis of biofilm structurea formed by various Streptococcus pneumoniae capsule serotypes
| Biofilm architecture group | Strain and serotype | Biomass (μm3/μm2) | Avg thickness (μm) | Maximum thickness (μm) | Substratum coverage | Roughness coefficient |
|---|---|---|---|---|---|---|
| I | Type 3 | 121.57 (4.9) | 139.92 (9.7) | 150.98 (6.4) | 0.403 (0.08) | 0.118 (0.09) |
| CHPB | 109.14 (6.3) | 103.70 (1.5) | 104.87 (2.2) | 0.471 (0.1) | 0.016 (0.005) | |
| Type 23 | 80.48 (0.04) | 100.76 (3.3) | 146.03 (3.5) | 0.050 (0.01) | 0.326 (0.01) | |
| ATCC (type 3) | 78.96 (2.2) | 88.54 (4.8) | 89.88 (5.2) | 0.259 (0.03) | 0.021 (0.006) | |
| II | Type 18 | 64.20 (3.4) | 69.72 (4.6) | 81.46 (2.7) | 0.185 (0.03) | 0.083 (0.002) |
| Type 6 | 64.07 (0.7) | 65.10 (1.7) | 65.58 (2.1) | 0.158 (0.04) | 0.023 (0.005) | |
| Type 9 | 58.00 (6.4) | 60.47 (6.5) | 72.09 (10.5) | 0.129 (0.04) | 0.110 (0.01) | |
| Type 11 | 54.59 (6.6) | 60.23 (9.3) | 64.82 (11.0) | 0.026 (0.008) | 0.088 (0.02) | |
| Type 14 | 44.90 (9.6) | 56.83 (14.2) | 110.55 (14.7) | 0.124 (0.08) | 0.331 (0.06) | |
| III | CHPA | 36.16 (3.9) | 39.40 (4.1) | 57.00 (10.3) | 0.060 (0.03) | 0.156 (0.03) |
| F3114 | 35.10 (2.3) | 47.75 (6.4) | 74.00 (2.8) | 0.031 (0.03) | 0.472 (0.02) | |
| Type 19 | 31.26 (5.3) | 36.70 (5.3) | 62.66 (4.4) | 0.041 (0.03) | 0.154 (0.01) | |
| W2938 | 27.49 (22.2) | 38.38 (24.1) | 77.50 (20.5) | 0.051 (0.03) | 0.526 (0.1) | |
| CHPC | 21.29 (5.3) | 29.58 (2.5) | 105.30 (2.5) | 0.036 (0.04) | 0.897 (0.1) |
a
Values are means of data from six z series image stacks for each strain or serotype taken at day 6. The numbers in parentheses are the standard deviations.