TABLE 3.
ODC and ADC activity in cellular extracts of Y. pestis speA+ speC+ (KIM6+) and polyamine-deficient mutants
| Enzyme | nmol CO2/min/mg ± SDa
|
μmol CO2/min/mg ± SDa
|
|||||
|---|---|---|---|---|---|---|---|
| speA+speC+ | ΔspeA | ΔspeC | ΔspeA ΔspeC | ΔspeA ΔspeC (pBAD/HisbADC) | Purified ADC | Purified ODC | |
| ADC | 20.86 ± 4.22 | 2.01 ± 0.85 | 15.36 ± 0.62 | 0.94 ± 0.35 | 26.14 ± 0.77 | 29.10 ± 7.93 | NDb |
| ODC | 8.98 ± 1.02 | 19.11 ± 4.92 | 0.67 ± 0.13 | 0.48 ± 0.39 | NDb | NDb | 1.89 ± 0.48 |
a
Values are the averages of three or more experiments.
b
ND, not determined.