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Effect of His6-Fnr* on in vitro transcription from PN. Multiple-round transcription reactions were carried out by using pJCD-PN, a template that produces a control mRNA of 105 nucleotides (C) and an mRNA from PN of 184 nucleotides (PN). Lanes 2 to 6, in vitro transcription reactions performed with 100 nM E. coli RNAP and 0, 1, 2.5, 5, and 10 nM of His6-Fnr*, respectively. Lane 1 shows a reaction without RNAP.
