FIG. 6.
AcpR and Fnr* control the expression of the bzd genes in Azoarcus sp. strain CIB. (A) Growth curves of Azoarcus sp. strain CIB (square), Azoarcus sp. strain CIBdacpR (circle), Azoarcus sp. strain CIBdacpR carrying plasmid pBBR1MCS-5acpR (triangle), and Azoarcus sp. strain CIBdacpR carrying plasmid pIZ-FNR* (pentagon) growing anaerobically in MC medium containing succinate (empty symbols) or benzoate (filled symbols). (B) Azoarcus sp. strain CIB (open blocks) and Azoarcus sp. strain CIBdacpR (filled blocks) carrying plasmid pBBR5PN (PN::lacZ) were grown anaerobically for 48 h in MC medium containing 0.4% (wt/vol) pyruvate (Pyr) or 0.4% (wt/vol) pyruvate plus 3 mM benzoate (Pyr+Bz). β-Galactosidase activity was measured as described in Materials and Methods. The results of one experiment are shown, and values were reproducible in three separate experiments with standard deviations of <10%.