TABLE 3.
Comparison of GeneChip-determined mRNA half-lives and real-time PCR-determined relative degradation
| Genea | Half-life (min)b | Fold decrease in mRNA titer at time (min)c:
|
|||
|---|---|---|---|---|---|
| 5 | 15 | 30 | 60 | ||
| gyrA | ≤5 | 9.8 (±0.8) | ND | ND | ND |
| purH | ≤5 | 15.3 (±6.5) | ND | ND | ND |
| hup | 30-60 | 1.2 (±0.5) | 1.8 (±0.3) | 7.8 (±2.1) | ND |
| SA1278 | >60 | 1.4 (±0.2) | 1.1 (±0.3) | 1.3 (±0.2) | 1.3 (±0.2) |
a
S. aureus strain N315 common gene name or locus.
b
GeneChip-determined mRNA half-life.
c
Real-time PCR-determined relative decrease in mRNA titer following 5, 15, 30, and 60 min of transcriptional arrest. ND, not determined.