FIG. 1.

Western and Northern analyses of MotP and MotS in the wild type, AB::Tn, AB::Tn-M, and ΔABΔPS. The cells were grown in 2× TY medium at 37°C. (A) Western analyses using antibodies against peptides corresponding to regions of MotP and MotS. The strain is indicated above each lane, with Wt representing the wild type. A quantitative imaging system, Pluor-S MAX (Bio-Rad), was used for detection and analysis of a chemiluminescence image. (B) Schematic diagram of the ccpA-motPS region of the B. subtilis chromosome indicating the probes used for Northern analyses. (C) Results of the Northern analyses, with the strain indicated above each lane and the probe used for the particular blot indicated below the panel. The expected sizes for ccpA mRNA and ccpA-motPS mRNA are indicated by a gray arrow and a black arrow, respectively.