Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Apr;188(7):2701–2705. doi: 10.1128/JB.188.7.2701-2705.2006

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, American Society for Microbiology

PMC Copyright notice

FIG. 2. — Expression of ccpA-lacZ and motPS-lacZ fusions in wild-type and ΔmotAB strains. (A) Schematic diagram of the integrated pMutin plasmid locus in the ccpA-motPS region. Each construct was inserted into the pMutin plasmid upstream of the stem-loop structure at the intergenic region of ccpA and motP. (B) Growth curves of strains. BR151MA (wild type), ○; W-ccpA-lacZ, ; W-motPS-lacZ, ▵; W-motPS-lacZ-M, . Growth in 2× TY medium at 37°C was monitored by A₆₀₀. (C) β-Galactosidase activity of the ccpA-lacZ and motPS-lacZ fusion constructs (indicated at the top of each panel) at different times during growth. Activity is expressed as nanomoles o-nitrophenyl-β-d-galactopyranoside hydrolyzed per minute per milligram protein. The strains used for the experiment are indicated below the panel. The error bars indicate the standard deviations of the values.

Inline graphic — Expression of ccpA-lacZ and motPS-lacZ fusions in wild-type and ΔmotAB strains. (A) Schematic diagram of the integrated pMutin plasmid locus in the ccpA-motPS region. Each construct was inserted into the pMutin plasmid upstream of the stem-loop structure at the intergenic region of ccpA and motP. (B) Growth curves of strains. BR151MA (wild type), ○; W-ccpA-lacZ, ; W-motPS-lacZ, ▵; W-motPS-lacZ-M, . Growth in 2× TY medium at 37°C was monitored by A₆₀₀. (C) β-Galactosidase activity of the ccpA-lacZ and motPS-lacZ fusion constructs (indicated at the top of each panel) at different times during growth. Activity is expressed as nanomoles o-nitrophenyl-β-d-galactopyranoside hydrolyzed per minute per milligram protein. The strains used for the experiment are indicated below the panel. The error bars indicate the standard deviations of the values.