TABLE 3.
Effect of ox bile on number of bacteria retained after 2 h in a polystyrene 96-well microtiter platea
| E. faecalis strain | No. of bacteria (106 cm−2) retained after 2 hb
|
|
|---|---|---|
| With ox bile | Without ox bile | |
| Heterogeneous | ||
| BS4126 | 11.5 ± 1.6 | 8.1 ± 1.4d |
| BS12297 | 7.8 ± 3.9 | 2.4 ± 0.9d |
| BS385 | 5.7 ± 0.8 | 2.5 ± 0.6d |
| BS1037 | 9.9 ± 0.9 | 2.0 ± 0.7d |
| Homogeneous | ||
| BS11297 | 1.0 ± 0.8c | 0.8 ± 0.2c |
| BS937 | 1.9 ± 1.4c | 0.4 ± 0.3c |
| Enriched subpopulation | ||
| BS385 | 4.1 ± 0.7e | ND |
a
Each plate contained 10 mM potassium phosphate with or without 50 mg/ml ox bile.
b
The results are the means ± standard deviations for three separate experiments performed in triplicate. ND, not determined.
c
P < 0.05, versus heterogeneous strains, Student's t test.
d
P < 0.05, versus without ox bile, Student's t test.
e
P < 0.05, versus BS385, Student's t test.