TABLE 2.
Donor strain | Phage | SaPI | Inducerb | Transduction titerc |
---|---|---|---|---|
JP44 | φ80α | SaPIbov1 | MC | 7.2 × 109 |
A | 2.7 × 107 | |||
P | 3.0 × 107 | |||
CL | 1.6 × 107 | |||
CE | 3.2 × 107 | |||
NI | 8.1 × 104 | |||
JP47 | φ11 | SaPIbov1 | MC | 2.1 × 106 |
A | 6.0 × 104 | |||
P | 5.8 × 104 | |||
CL | 4.4 × 104 | |||
CE | 5.6 × 104 | |||
NI | 1.7 × 103 | |||
JP50 (recA mutant) | φ11 | SaPIbov1 | MC | <10 |
A | <10 | |||
NI | <10 | |||
JP85 [JP83 LexA (G94E)] | φ80α | SaPIbov1 | MC | 5 × 105 |
A | 4 × 104 | |||
NI | 2.3 × 103 | |||
JP52 | φ11 cIG131E | SaPIbov1 | MC | <10 |
A | <10 | |||
NI | <10 | |||
RN8667 | φ80α | SaPI1 | A | 2.1 × 105 |
P | 1.6 × 105 | |||
CL | 2.2 × 105 | |||
CE | 2.9 × 105 | |||
NI | 6.0 × 103 |
Results from a representative experiment are shown.
MC, mitomycin C (2 μg/ml); A, ampicillin (10 μg/ml); P, penicillin G (10 μg/ml); CL, cloxacillin (10 μg/ml); CE, ceftriaxone (10 μg/ml); NI, not induced.
Number of transductants per milliliter of induced culture, using RN4220 as the recipient strain.