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. 2006 Apr;188(7):2446–2453. doi: 10.1128/JB.188.7.2446-2453.2006

FIG. 4.

FIG. 4.

VqmA regulates the hapR promoter directly. (A) hapR-lacZ expression in E. coli. MC4100 with either a vector control, a plasmid constitutively expressing wild-type VqmA, or a plasmid expressing VqmA lacking its DNA binding domain (ΔHTH) was inoculated 1:100 into LB in the presence of 0.1 μM IPTG and grown at 37°C for 2 h (white bars) or 6 h (gray bars). Results are representative of three experiments. Error bars, standard deviations. (B) Gel retardation assays using purified MBP-VqmA proteins. Lanes 1 to 4, labeled hapR promoter region; lanes 5 and 6, labeled hapR promoter region without upstream sequences. Lanes 1 and 5, labeled DNA only; lanes 2 and 6, 0.5 μg MBP-VqmA; lane 3, 0.5 μg MBP-VqmA with 0.1 μg unlabeled DNA; lane 4, 0.5 μg MBP-VqmA(ΔHTH). wt, wild type.