FIG. 1.

Construction of lodA mutant strains. Plasmid pFSVK-SS was created as shown. After integration of this plasmid into the genome of M. mediterranea by homologous recombination, two different genomic layouts were possible (A and B). They were distinguished by PCR using primers KMREV and MAREV5, obtaining PCR products of ca. 1,800 bp and 1,400 bp, respectively. Mutant strains with construction A, such as SB1, are nonproducers of marinocine. In contrast, mutant strains with construction B are producers of marinocine.