FIG. 2.

Analysis of steady-state levels of pixA mRNA by RT-PCR. (A). Total RNA was isolated from the parental X. nematophila strain at different times during growth. Lane 1, early exponential phase; lane 2, mid-exponential phase; lane 3, late exponential phase; and lane 4, stationary phase. PCR primers directed to internal sequences of pixA were used in the RT-PCR. (B). Internal control reaction using total RNA as above and primers directed to the 16S rRNA.