FIG. 2.

(A) Chromosomal arrangement of the ddlA-murF operon region in COL and COLspacmurF strains. The primers used for the verification of correct insertion of pMGPI into the chromosome are identified. (B) PCR fragments amplified from COL and COLspacmurF chromosomal DNA using combinations of primers p1 to p6 (Table 2). Lanes 1, 10, and 11, 1-kb Plus DNA ladder; lanes 2 to 9, PCR products amplified using Tth DNA polymerase; lanes 12 and 13, PCR products amplified using Pfu Turbo and under long-range PCR conditions (see Materials and Methods).