FIG. 1.

Phenotype analysis of A. tumefaciens strains K588 (square) and A6 (triangle). The assays were performed with (solid symbol or bar) or without (open symbol or bar) octopine. (A) Time course of growth of the two strains in BM minimal medium. Bacterial overnight culture was diluted in BM medium to same population density (OD₆₀₀ = 1.0), inoculated into BM medium at a 1:100 ratio, and incubated with shaking (200 rpm) at 28°C. (B) AHL production by A. tumefaciens strains in liquid cell culture. The culture conditions were the same as above. Note, the data for A6 with and without octopine were identical, and as a result, solid triangles overlapped with open triangles. (C) AHL production by A. tumefaciens strains on BM medium agar plate. The procedures for inoculation and bioassay were described in Materials and Methods. (D) Ti plasmid conjugative transfer efficiency of A. tumefaciens strains. Induction of donor cells was done on solid agar medium as described in Materials and Methods. Values are presented as means ± standard deviations of results from at least three independent repeats.