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Sequence alignment of three TraM homologues used in this study. Alignment of amino acid sequences was performed using the computational program Clustalw (http://sbcr.bii.a-star.edu.sg/clustalw/). The key amino acid residues showing strong influence on inhibition activity against TraR-mediated gene activation, identified in a previous study (29), were indicated by a triangle above the sequence. TraMK588 differs from TraM in only one amino acid (L54P).
