FIG. 7.
Inhibitory effect of TraM2 on DNA binding to TraR by gel mobility assay. The inhibition potency of TraM2 from strain A6 (A) is compared with that of TraMR10 (B). Concentrations of proteins (TraR, TraM, and TraM2) in the reaction mixtures are indicated above the nondenaturing gels (8%). Positions of the IR-labeled tra box in both free and complexed with TraR forms are indicated. At 16 μM, TraM2 displaced TraR completely from the tra box, compared to a lower concentration (∼2 μM) of TraM. −, not included in the reaction mixture.