TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant phenotypes and/or characteristicsa | Source or reference |
|---|---|---|
| A. tumefaciens | ||
| NT1(traR tra::lacZ749) | C58C1RS harboring pJM749 and pSVB33, AHL bioassay indicator strain | 25 |
| K588 | C58C1 harboring octopine type Ti plasmid pTiB6S3Trac, Eryr, Chlr | A. Kerr |
| A6 | Wild-type octopine strain containing pTiA6, Trai | A. Kerr |
| Ach5 | Wild-type octopine strain containing pTiAch5, Trai | C. Fuqua |
| R10 | Wild-type octopine strain containing pTiR10, Trai | C. Fuqua |
| B6 | Wild-type octopine strain containing pTiB6, Trai | C. Fuqua |
| B6S3 | Wild-type octopine strain containing pTiB6S3, Trai | C. Fuqua |
| ATCC 15955 | Wild-type octopine strain containing pTi15955, Trai | C. Fuqua |
| C58 | Wild-type nopaline strain containing pTiC58, Trai | S. Q. Pan |
| C58C1RS | Derivative of strain C58, pTi−, Rifr, recipient strain for Ti plasmid conjugative transfer | S. Q. Pan |
| K749 | Derivative of strain C58, pTi−, pAT−, Rifr | A. Kerr |
| Ach5C3 | Derivative of strain Ach5, Ti plasmid cured, Rifr | S. K. Farrand |
| A6(pTiA6traMK588) | Derivative of strain A6 where the traM gene was replaced with the traM from strain K588 | This study |
| C58C1RS(pTiA6traMK588) | Derivative of strain C58C1RS with the Ti plasmid donated by A6(pTiA6traMK588) | This study |
| C58C1RS(pTiK588) | Derivative of strain C58C1RS harboring pTiK588 of K588 | This study |
| Ach5C3(pTiA6traMK588) | Derivative of strain Ach5C3 with the Ti plasmid donated by A6(pTiA6traMK588) | This study |
| Ach5C3(pTiK588) | Derivative of strain Ach5C3 harboring pTiK588 of K588 | This study |
| E. coli | ||
| DH5α | supE44 ΔlacU169(φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 λpir | Laboratory collection |
| S17-1 | pro res− mod+ integrated copy of RP4, mob+ | A. Kerr |
| Plasmids | ||
| pLAFR3 | IncP, broad-host-range cosmid vector, Tetr | 28 |
| pAW19 | f1(+)ori lacZα of pBluescript II (SK+), sacB, Sucr | S. K. Farrand |
| pLA-traMA6-PT | pLAFR3 harboring the coding region of traM amplified from pTiA6; the gene is under the control of the lac promoter of the vector, Tetr | This study |
| pLA-traM2 | pLAFR3 harboring the coding region of traM2 that is placed under the control of the lac promoter of the vector, Tetr | This study |
| pJZ358 | Expression construct for TraRR10 expression | 41 |
| pGC-traM2 | Expression construct for TraM2 expression | This study |
| pBK129 | Cosmid clone containing about 26-kb tra region from pTiK588 | This study |
| pAW19-traMK588 | pAW19 containing the traM coding sequences and its flanking region from pTiK588 | This study |
| pAW-traM2d | pAW19 containing the flanking regions of traM2 from strain A6 | This study |
a
Abbreviations: Trac, constitutive in AHL production and Ti plasmid conjugative transfer; Trai, AHL production and Ti plasmid conjugative transfer are inducible by opine inducer; Ampr, ampicilin resistant; Kanr, kanamycin resistant; Tetr, tetracycline resistant; Rifr, rifampicin resistant; Eryr, erythromycin resistant; Chlr, chloramphenicol resistant; Sucr, sucrose resistant.