FIG. 1.

Disruption of a novel gene named mbaA in V. cholerae N16961 leads to increased biofilm formation. (A) Crystal violet-stained borosilicate tubes and quantification of the DMSO-solubilized dye are shown for the indicated strains after 30 h of growth in LB broth at room temperature. All assays were performed in triplicates. (B) Genetic organization of the mbaA region on the chromosome of V. cholerae N16961. The a, b, mbaA, c, and d open reading frames correspond to loci VC0705, VC0704, VC0703, VC0702, and VC0701, respectively, based on the DNA sequences obtained from The Institute for Genomic Research microbial genome database. Open reading frames a, mbaA, and c might constitute an operon. The Tn10 insertion in mbaA and the extent of the in-frame deletion created in mbaA are indicated.