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The production of EPS is required for biofilm formation in the absence of mbaA. WT, vsp59::pGp704, and ΔmbaA-vps59::pGp704 mutant cells of V. cholerae N16961 were incubated in LB broth at room temperature for 40 h. Phase contrast micrographs of biofilms formed on borosilicate coverslips are shown in the left panels. Crystal violet-stained borosilicate tubes are shown in the right panels.
