FIG. 4.

The absence of mbaA leads to alterations in the biofilm architecture. WT and gfp-tagged ΔmbaA mutant cells of V. cholerae N16961 were incubated with borosilicate coverslips in LB broth at room temperature. Biofilm formation on the coverslips was observed at the indicated times. Shown are phase contrast micrographs of biofilms formed after 8 (A) and 40 h (B) and confocal scanning laser micrographs of biofilms formed after 65 h of incubation (C). Micrographs represent optical sections in the x-z plane, with the substratum located at the bottom. Bar, 5 μm.