FIG. 2.

Preliminary master gel of B. subtilis coat preparations and MALDI analysis. (A) pH 3 to 10 gel of B. subtilis coat preparations. Sample was solubilized in 10% LDS buffer. The solution was diluted in an excess of standard solubilization buffer containing 2% C8φ and 1% TX-100 and resolved by IEF. The second dimension was carried out on an SDS-11% polyacrylamide gel and silver stained (56). Spots identified by peptide mass fingerprinting are circled and numbered, and the identifications are presented in Table 2. (B) MALDI-MS spectrum for spot 1.