TABLE 2.
Proteins identified by MALDI-TOF MS from 2D separation of B. subtilis spore coat fractions
| Spot no. | MOWSE score | No. of peptides matched | MWb/pI | Name | Function |
|---|---|---|---|---|---|
| 1 | 2.15E+06 | 11 | 58,499.3/5.91 | CotA | Multicopper oxidase |
| 2 | 5.50E+05 | 13 | 48,637.0/5.72 | YaaH | Unknown protein |
| 3 | NAa | 9 | 48,534.4/7.5 | YhbA | Unknown protein |
| 4 | 1.14E+06 | 10 | 58,499.3/5.91 | CotA | Multicopper oxidase |
| 5 | 8.49E+03 | 6 | 50,085.0/6.12 | YvdP (CotQ) | Similar to coat protein CotQ |
| 6 | 8.16E+02 | 5 | 53,503.9/5.41 | YopQ | Unknown protein |
| 7 | NA | 8 | 129,334/5.44 | YirY | Unknown protein |
| 8 | NA | 8 | 37,382.4/5.8 | CggR | Transcriptional regulator |
| 10 | 3.44E+04 | 6 | 30,559/6.8 | YisY | Unknown protein |
| 11 | NA | 7 | 21,695.8/5.1 | CotJC | Coat protein |
| 12 | 1,453 | 4 | 20,977/4.4 | CotE | Morphogenetic coat protein E |
| 13 | 7,589 | 5 | 20,977/4.4 | CotE | Morphogenetic coat protein E |
| 14 | 6.26E+05 | 8 | 20,977/4.4 | CotE | Morphogenetic coat protein E |
| 16 | NA | 5 | 18,728/5.02 | CotY | Coat protein Y |
| 17 | NA | 5 | 15,857/5.01 | YwqH | Unknown protein |
| 18 | NA | 5 | 14,785/9.4 | CotC | Coat protein C |
| 19 | NA | 5 | 14,785/9.4 | CotC | Coat protein C |
| 20 | NA | 5 | 14,785/9.4 | CotC | Coat protein C |
| 21 | 5.62E+03 | 6 | 11,562.5/6.28 | YnzH (CotU) | Coat protein |
a
NA, identifications were made with the MASCOT algorithm or with the Micromass Protein Lynx software package.
b
MW, molecular weight.