TABLE 2.
β-Galactosidase activities of B. pertussis bug-lacZ recombinants
| Target gene | β-Galactosidase activity (Miller units)a in:
|
||
|---|---|---|---|
| BGb | BG + MgSO4 | BG + NA | |
| bug1 | 40 ± 1 | 33 ± 7 | 36 ± 9 |
| bug4 | 77 ± 9 | 107 ± 22 | 132 ± 27 |
| bug9 | 2,863 ± 997 | 2,639 ± 603 | 1,998 ± 240 |
| bug20 | 205 ± 39 | 292 ± 97 | 274 ± 61 |
| bug24 | <5c | <5 | <5 |
| bug43 | 411 ± 109 | 343 ± 118 | 346 ± 53 |
| bug60 | 22 ± 4 | 49 ± 5 | 31 ± 5 |
| bug64 | 85 ± 40 | 107 ± 37 | 93 ± 26 |
| bug71 | 1,193 ± 61 | 2,178 ± 292 | 2,964 ± 399 |
| bug73 | 207 ± 15 | 490 ± 50 | 496 ± 60 |
| bug79 | 323 ± 161 | 267 ± 171 | 209 ± 52 |
a
Data are means ± standard deviations from 3 to 5 independent experiments.
b
BG, Bordet Gengou medium; NA, 20 mM nicotinic acid.
c
Below the limit of detection.