FIG. 2.

Octanucleotides corresponding to novel joints. As a convenient way to show the relative positions of the sequences, the upper strand of the wild-type pyrE gene is drawn annealed to the octanucleotide in the lower strand that represents the novel joint of the corresponding deletion. This method of depiction is that used in other deletion studies (11, 12, 16, 30) and is not a proposal of a specific mechanism. Sequences remaining after deletion are shown in bold type, as for Table. 1. Numbers with vertical lines indicate the nucleotide position in each strand with respect to the pyrE coding sequence; the remaining numbers indicate the sizes of the loops shown.