TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| P. aeruginosa | ||
| K767 | PAO1 prototroph | N. Gotoh, Kyoto Pharmaceutical University |
| K1120 | PAO1 ΔaphA | N. Gotoh, Kyoto Pharmaceutical University |
| K1203 | K1120 ΔpvdD | A. Meldrum, unpublished |
| K2100 | K1120 ΔfpvI | This study |
| K2102 | K1203 ΔfpvI | This study |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 2 |
| S17-1 | thi pro hsdR recA Tra+ | 43 |
| Plasmids | ||
| pEX18tc | Broad-host-range gene replacement vector; Tcr | 15 |
| pK18mobsacB | Broad-host-range gene replacement vector; Kanr | 40 |
| pRK415 | Broad-host-range, low-copy-number cloning vector carrying MCS downstream of Plac; Tcr | 16 |
| pMP190 | Broad-host-range, low-copy-number lacZ fusion vector; Camr Smr | 44 |
| pEJB3 | pMP190::fpvA-lacZ; Camr | E. J. Blouin, unpublished |
| pAR001 | pRK415::fpvI | This study |
| pAR002 | pEX18tc::ΔfpvI | This study |
| pAR003 | pK18mobsacB::ΔfpvI | This study |
| pAR005 | pRK415 digested with DraI, religated (ΔDraI) | This study |
a
Camr, chloramphenicol resistance; Kanr, kanamycin resistance; Tcr, tetracycline resistance; Smr, streptomycin resistance; MCS, multiple cloning site; Plac, lac promoter.