TABLE 2.
Influence of FpvI on fpvA-lacZ expressiona
| Strain | Relevant phenotype | β-Galactosidase activity (Miller units) withb:
|
|
|---|---|---|---|
| No supplementation | Pyoverdine supplementation | ||
| K1120 | FpvI+ | 1,400 | —c |
| K2100 | FpvI− | 256 | — |
| K2100 (pAR001) | FpvI+ | 1,455 | — |
| DH5α (pAR001) | FpvI+ | 106 | — |
| DH5α (pAR005) | FpvI− | 3 | — |
| K1203 | FpvI+ Pvd−d | 616 | 1,749 |
| K2102 | FpvI− Pvd− | 370 | 298 |
a
P. aeruginosa and E. coli strains harboring the fpvA-lacZ vector pEJB3 were cultured to log phase in antibiotic-supplemented iron-limited medium with or without the addition of 100 μg of pyoverdine/ml and assayed for β-galactosidase activity.
b
Data are a representation of experiments performed in triplicate.
c
—, not done.
d
Pyoverdine deficient owing to a deletion in the pvdD gene.