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. 2003 Feb;185(4):1326–1337. doi: 10.1128/JB.185.4.1326-1337.2003

FIG. 2.

FIG. 2.

Locations of sister copies of various regions of the chromosome. Strains producing Spo0J-GFP, LacI-GFP, LacI-CFP, or Rtp-YFP were grown at 30°C in defined minimal medium. Only cells with two foci of the region of interest were analyzed. The distance from each focus to the same cell pole was measured from images of live cells in exponential growth (Materials and Methods) and is plotted on the x axis, and cell length is plotted on the y axis. Cell length and the midcell positions are indicated by solid lines. Cell quarter positions are indicated by dotted lines. The number of cells analyzed (n) is indicated in the lower right corner of each panel. One focus is indicated with red open circles and the other with blue crosses. (A) Position of Spo0J-GFP bound to endogenous parS sites (strain PSL10). (B, C) Position of sister origin regions (359°) visualized with LacI-GFP bound to lac operator arrays in spo0J+ (strain DCL696) and spo0J mutant (strain DCL705) cells. (D, E) Position of Spo0J-GFP bound to a parS array (D, strain PSL25) or LacI-CFP bound to a lac operator array (E, strain KPL718) inserted at 90°. (F) Position of Spo0J-GFP bound to a parS array inserted at 210° (strain PSL27). (G, H) Position of Spo0J-GFP bound to a parS array (G, strain DCL631) or LacI-CFP bound to a lac operator array (H, strain KPL686) inserted at 181°. (I) Position of Rtp-YFP bound to endogenous ter sites (strain IRN424). (J, K) Position of Spo0J-GFP bound to a parS array (J, strain PSL23) or LacI-CFP bound to a lac operator array (K, strain KPL716) inserted at 270°.